WEED CONTROL Herbicide Residue Herbicide Persistence The persistence of phytotoxic levels of a herbicide for more than one year can be a problem with some of the herbicides used in North Dakota. Herbicide residues are most likely to occur following years with unusually low rainfall because chemical and microbial activity needed to degrade herbicides are limited in dry soil. Crop damage from herbicide residues can be minimized by applying the lowest herbicide rate required for good weed control, by using band rather than broadcast applications, and by moldboard plowing before planting the next crop. Moldboard plowing reduces phytotoxicity of some herbicides by diluting the herbicide residue in a large volume of soil. Moldboard plowing is effective in reducing the residual effects of trifluralin, Sonalan, Prowl, Nortron SC, atrazine, and Lexone/Sencor. Herbicide residues often can be detected by bioassay. A soil sample representative of the whole field must be obtained by sampling at many places to the depth of the tillage layer. Also, a sample of soil known to be free of herbicide residues must be obtained from near the treated field to serve as the untreated check. The samples should be dried and the clods broken so that the largest particles are no larger than a wheat kernel. Prepare at least two samples each of the untreated check soil and the test soil in pots or other containers with holes in the bottom for water drainage. The crop to be grown in the field should be used as one bioassay species. Preparing extra pots and testing a more susceptible species may be helpful in detecting residues. Plant in each pot 12 seeds of large-seeded crops like corn or soybean, or 20 seeds of small-seeded crops like cereals or flax. Water the soil for germination and plant growth as needed, but do not over-water. When the plants are about 2 inches tall, thin to about six large-seeded or 12 small-seeded uniform seedlings in each container. The containers should be kept at about 70 to 75 F, and in direct sunlight. Observe the plants in the untreated check and test samples for two to three weeks after emergence. Some tangible measurements such as plant height and leaf length can be taken for evaluation, along with visual observation of abnormalities. Symptoms of some herbicides, like atrazine and Lexone/Sencor do not develop until several days after emergence. The soil should be washed from the roots to observe root growth, especially for dinitroaniline herbicides such as Prowl, trifluralin and Sonalan. Window bioassay does not provide accurate information on Ally or Amber carryover.